Welcome to the RII Division FACS Orientation & Training page

Please follow these steps to gain access to our core:

1. Watch both Canto II & Fortessa Orientation videos
2. Complete BD Biosciences e-Learning Courses
3. Shadow lab members and perform supervised operation of the cytometers for a minimum of 2 weeks
4. After completing all of the above, fill out the ATTESTATION FORM and send to: riifc@bwh.harvard.edu
5. Register an account with the MGB Core Management System
6. Schedule 1-hour supervised training for your first experiment

Orientation Videos: Canto II & Fortessa

BD Biosciences e-Learning Courses

• Introduction to Flow Cytometry

1. 1. Overview (05:01)
   2. Fluidics (3:30)
   3. Optics (07:48)
   4. Electronics (07:00)
   5. Optical Measurement (09:48)
   6. Data Analysis (08:54)

• BD FACSDiva™ Software Basics Training

1. 1. BD FACSDiva Software Overview (self-paced)
   2. Data Display in BD FACSDiva Software (self-paced)
   3. Using the Worksheet Window in BD FACSDiva Software (self-paced)